This repository features workflow for data analysis of DroNc-seq Data (Habib et al., 2017). dropseq_minimal.Rmd takes you through minimal example of processing in R,mainly using Seurat. The dropseq.Rmd and the associated PDF document then give full recipe of bioinformatical workflow starting from FASTQ files and going all the way to plotting in R.
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To run the minimal example, you will need to download some files.
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The script for the full analysis cannot be executed due to the volume of necessary data. Instead, it serves as a guide for developing a workflow of your own.
Described in the PDF.
- Martin Holub - web
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authors of Drop-seq Tools and dropSeqPipe for the developed and published code
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ETH for computing resources available to students via Euler.
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Prof. Robinson and his students and co-workers for organizing the STA426 course