Particle tracing in 3D by holographic data using LODESTAR

[avi-xd]

Hi, I have been using the LODESTAR model to get the traces of particles for my off-axis Holographic data. I used the model and got results quite accurately on the X-Y plane. However, on the Z axis, the model could not predict very efficiently, as it gave sudden jumps (spikes) between the traces (I am uploading the output).

But then I checked with the example uploaded by you guys in 07.track_3D_holography.ipynb

I got the same results as in the paper https://www.nature.com/articles/s41467-022-35004-y. But when I plotted the traces in 3D, they also had similar jumps (pics attached below).

In 2D (x-y plane), result is

and similar result in 3D looks like

I am not able to figure out what causes these sudden spikes in the results and how to tackle it and get better results. Any help or key insight will be highly appreciated.

I can provide the exact code I have used if needed. Thanks

[avi-xd]

@BenjaminMidtvedt any inputs here? Thanks